Cationic-Enhanced Spectrofluorimetric Method for Determination of Selective Serotonin Reuptake Inhibitor Duloxetine Hydrochloride in its Dosage Forms

Duloxetine hydrochloride is chemically, N-methyl-3napthalen-1-oxy-3-thiophen-2-yl-1-amine. Duloxetine hydrochloride (Fig. 1) is a new selective serotonin and norepinephrine reuptake inhibitor used for major depressive disorders. It is also indicated for the management of neuropathic pain associated with diabetic peripheral neuropathy. Duloxetine hydrochloride is considered among the most important antidepressant drugs therefore, the development of reliable rapid and accurate procedures for this active ingredient quantification is welcomed. Several analytical methods have been reported for determination of duloxetine hydrochloride in its pure form, pharmaceutical preparations and biological fluids, where duloxetine hydrochloride is determined officially in any pharmacopoeia. These methods include high-performance liquid chromatography, liquid chromatography coupled with mass spectrometry, high performance thin layer chromatography, spectrophotometry, capillary zone electrophoresis, screen printed potentiomerty and spectrofluorimetry. The reported spectrofluorometric method is based on the investigation of native fluorescence of duloxetine hydrochloride in a concentration range 0.02-0.4 μg/mL in 0.05 mol/L acetic acid having excitation at 225 nm and emission at 340 nm. Although, Cationic-Enhanced Spectrofluorimetric Method for Determination of Selective Serotonin Reuptake Inhibitor Duloxetine Hydrochloride in its Dosage Forms